Tipo de Articulo: Artículos Originales

Titulo: Influencia del fosfato β-triclcico de diferentes formas geomtricas en la morfologa de la regeneracin del defecto experimental del tejido seo compacto

Title: Influence of β-tricalcium phosphate of different geometric shape on the morphology of regeneration of experimental defect of compact bone tissue

Actual. Osteol 13(1):17:27, 2017

Autor(es): Alexsey Korenkov


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Objetivo: Comparar el proceso de cicatrizacin de un defecto del tejido seo compacto tras la implantacin de materiales osteoplsticos a base de fosfato β-triclcico ("ChronOS" and "Calc-i-oss") que difieren segn el fabricante en la forma geomtrica y estructura microscpica. El estudio fue realizado en 48 ratones Wistar machos en los cuales se produjo, en el tercio medio de la difisis del fmur, un defecto perforado de 2,5 mm de dimetro, el cual fue llenado con el material "ChronOS" (block, Synthes, Switzerland) en un grupo y con "Calc-i-oss" (granules, Degradable Solutions Dental, Switzerland) en el segundo grupo. El sector del defecto fue evaluado en los das 60 y 120 por microscopa ptica y por microscopa electrnica de barrido. Resultados: independientemente de la forma geomtrica y la estructura microscpica, ambos materiales osteoplsticos mostraron alta biocompatibilidad, propiedades osteoconductivas y buena integracin con el tejido seo regenerado. La estructura microscpica del fosfato β-triclcico ("ChronOS") puede afectar significativamente a la estructura microscpica del tejido seo regenerado, que se manifiesta en su forma geomtrica. Adicionalmente, se observ que ambos materiales osteoplsticos "ChronOS " y "Calc-i-oss" mostraron valores similares de resorcin y reemplazo por tejido seo, cuya relacin al 60 da del experimento fue de 22.551.25 a 77.451.25 y 25.722.06% a 74.282.06%, y a los 120 das de 17.651.09 a 82.351.09 y de 18.311.54% a 81.691.54% resspectivamente.

Palabras clave: ratas, hueso, fosfato β-triclcico, osteognesis.

Purpose: to compare the healing process of a defect of compact bone tissue after the implantation of osteoplastic materials based on β-tricalcium phosphate ("ChronOS" and "Calc-i-oss"), which differ by manufacturer, geometrical shape and microscopic structure. Methods: the experiment was performed on 48 white male Wistar rats. In the middle third of the diaphysis of the femur we produced a perforated defect of 2.5 mm diameter in the medullary canal, which in the animals of the first group was filled with the osteoplastic material "ChronOS" (block, Synthes, Switzerland), and in the animals of the second group - with "Calc-i-oss" (granules, Degradable Solutions Dental, Switzerland). Fragments of the injured bones were studied on the 60th and 120th day by light microscopy with morphometry and by scanning electron microscopy. Results: it was found that regardless the geometric shape and the microscopic structure, both osteoplastic materials show high biocompatibility, osteoconductive properties, good integration with bone tissue of the regenerate, and that the microscopic structure of β-tricalcium phosphate ("ChronOS") may significantly affect the microscopic structure of bone tissue of the regenerate, which manifests itself in the specificity of its geometric shape. It was noticed that osteoplastic materials "ChronOS" and "Calc-i-oss" almost at the same rate were subjected to resorption and replacement by the bone tissue, the ratio of which on the 60th day of the experiment was 22.551.25 to 77.451.25 and 25.722.06% to 74.282.06%, and on the 120th day 17.651.09 to 82.351.09 and 18.311.54% to 81.691.54%.

Key words: rats, bone, β-tricalcium phosphate, reparative osteogenesis.

Propietario: Asociacin Argentina de Osteologa y Metabolismo Mineral (AAOMM)
Domicilio legal: 9 de Julio 1324, (2000) Rosario, Santa Fe, Argentina.
Editores responsables: Dra. Luisa Plantalech - Dra. Virginia Massheimer

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